Journal: Animals : an Open Access Journal from MDPI

Article Title: Sulforaphane Enhanced Proliferation of Porcine Satellite Cells via Epigenetic Augmentation of SMAD7

doi: 10.3390/ani12111365

Figure Lengend Snippet: SFN increased global histone acetylation in PSCs. ( A ): The HDAC activity in Ctrl and SFN treated PSCs; ( B ): HDAC family members’ relative mRNA expression levels; ( C , D ): Western blot results of acetylated histone H3 and H4 in PSCs treated with Ctrl and SFN, and Coomassie-Brilliant Blue staining as loading control. Data are shown as the mean ± SE and assays were done in triplicates. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The primary antibodies utilized in this work included these for acetyl-histone H4 (06-866, Millipore, Burlington, MA, USA), acetyl-histone H3 (06-599, Burlington, MA, USA), SMAD7 (sc-11392, Santa Cruz, Dallas, TX, USA), and TGFB1 (sc-146, Santa Cruz, Dallas, TX, USA).

Techniques: Activity Assay, Expressing, Western Blot, Staining, Control